pISV-DsRed1-nopP
(Plasmid
#171025)
-
PurposeAgrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 171025 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepISV-DsRed1
-
Vector typePlant Expression
-
Selectable markersBasta ; DsRed
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namenopP
-
SpeciesSinorhizobium sp. NGR234
- Promoter 2 X EN 35S
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pISV-DsRed1-nopP was a gift from Christian Staehelin (Addgene plasmid # 171025 ; http://n2t.net/addgene:171025 ; RRID:Addgene_171025)