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pCDH-EF1-Large BiT - SNAP-tag - Beta-actin
(Plasmid #171019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 7657
  • Total vector size (bp) 9819
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LargeBit-SNAPtag- Beta-actin
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2163
  • Promoter EF1
  • Tags / Fusion Proteins
    • LargeBiT (N terminal on insert)
    • Beta actin (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGCCGGCTCTCCACGC
  • 3′ sequencing primer gcttcggccagtaacgtta
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Actin-mRFP-PAGFP was a gift from Guillaume Charras & Tim Mitchison (Addgene plasmid # 62382 ; http://n2t.net/addgene:62382 ; RRID:Addgene_62382)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-EF1-Large BiT - SNAP-tag - Beta-actin was a gift from Angus Johnston (Addgene plasmid # 171019 ; http://n2t.net/addgene:171019 ; RRID:Addgene_171019)

  • For your References section:

    Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay. Teo SLY, Rennick JJ, Yuen D, Al-Wassiti H, Johnston APR, Pouton CW. Nat Commun. 2021 Jun 17;12(1):3721. doi: 10.1038/s41467-021-23997-x. 10.1038/s41467-021-23997-x PubMed 34140497
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