pCDH-EF1-TfR20-SNAP-IRES-Puro
(Plasmid #171018)

• Purpose: Lentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cells
• Depositing Lab: Angus Johnston
• Publication: FitzGerald et al Nat Commun. 2020 Sep 8;11(1):4482. doi: 10.1038/s41467-020-18082-8.

Backbone

• Vector backbone: pCDH
• Backbone manufacturer: System Biosciences
• Backbone size w/o insert (bp): 7659
• Total vector size (bp): 10543
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Transferrin receptor (Human) - SNAP-tag
• Alt name: NM_003234
• Species: H. sapiens (human)
• Insert Size (bp): 2886
• Entrez Gene: TFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
• Promoter: EF1
• Tags / Fusion Proteins:
  • SNAPtag (C terminal on insert)
  • TfR (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CAGCCGGCTCTCCACGC
• 3′ sequencing primer: gcttcggccagtaacgtta

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mCherry-TFR-20 was a gift from Michael Davidson (Addgene plasmid # 55144 ; http://n2t.net/addgene:55144 ; RRID:Addgene_55144)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.08.20.258350v1 for bioRxiv preprint.