Skip to main content
Addgene

pCDH-EF1-TfR20-SNAP-IRES-Puro
(Plasmid #171018)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCDH
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 7659
  • Total vector size (bp) 10543
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Transferrin receptor (Human) - SNAP-tag
  • Alt name
    NM_003234
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2886
  • Entrez Gene
    TFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
  • Promoter EF1
  • Tags / Fusion Proteins
    • SNAPtag (C terminal on insert)
    • TfR (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGCCGGCTCTCCACGC
  • 3′ sequencing primer gcttcggccagtaacgtta
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mCherry-TFR-20 was a gift from Michael Davidson (Addgene plasmid # 55144 ; http://n2t.net/addgene:55144 ; RRID:Addgene_55144)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-EF1-TfR20-SNAP-IRES-Puro was a gift from Angus Johnston (Addgene plasmid # 171018 ; http://n2t.net/addgene:171018 ; RRID:Addgene_171018)

  • For your References section:

    A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells. FitzGerald LI, Aurelio L, Chen M, Yuen D, Rennick JJ, Graham B, Johnston APR. Nat Commun. 2020 Sep 8;11(1):4482. doi: 10.1038/s41467-020-18082-8. 10.1038/s41467-020-18082-8 PubMed 32901011
Related items:
Commonly requested with: