pMCB619
(Plasmid #171011)

• Purpose: CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).
• Depositing Lab: Alice Ting
• Publication: Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.

Backbone

• Vector backbone: pCRISPRia-v2
• Backbone size w/o insert (bp): 9000
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin ; BFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6:sgRNA_Puro-T2A-BFP
• gRNA/shRNA sequence: Unspecified
• Species: H. sapiens (human)
• Insert Size (bp): 50
• Promoter: mU6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pMCB619.gb
• A portion of this plasmid was derived from a plasmid made by: Michael Bassik
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry