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TMED3-hLOV-TEVcs-GAL4bd-VP64-V5
(Plasmid #170996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170996 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX208
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5
  • Species
    Synthetic
  • Insert Size (bp)
    2000
  • Entrez Gene
    TMED3 (a.k.a. C15orf22, P24B, p24g4, p24gamma4, p26)
  • Promoter Ef1-alpha
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TMED3-hLOV-TEVcs-GAL4bd-VP64-V5 was a gift from Alice Ting (Addgene plasmid # 170996 ; http://n2t.net/addgene:170996 ; RRID:Addgene_170996)

  • For your References section:

    An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens. Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, Weissman JS, Carr SA, Bassik MC, Ting AY. Elife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142. 10.7554/eLife.69142 PubMed 34414886
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