pUCSh3-mRFP
(Plasmid #170983)

• Purpose: Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.
• Depositing Lab: Christopher Reisch
• Publication: Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.1021/acssynbio.3c00065.

Backbone

• Vector backbone: pUC
• Backbone size w/o insert (bp): 5405
• Total vector size (bp): 6145
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mRFP
• Alt name: monomeric red fluorescent protein
• gRNA/shRNA sequence: N/A
• Promoter: J23119

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ggggttccgcgcacatttc
• 3′ sequencing primer: gacgaaagggcctcgtga

Resource Information

• Supplemental Documents:
  • pUCShDon3-mrfp.gbk
• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid #127924 - pDonor_ShCAST_kanR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry