pUCSh2-mRFP
(Plasmid
#170982)
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PurposeEncodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170982 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
- Backbone size w/o insert (bp) 4879
- Total vector size (bp) 5739
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRFP
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Alt namemonomeric red fluorescent protein
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gRNA/shRNA sequenceN/A
- Promoter J23119
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ggggttccgcgcacatttc
- 3′ sequencing primer gacgaaagggcctcgtga (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid #127924 - pDonor_ShCAST_kanR
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUCSh2-mRFP was a gift from Christopher Reisch (Addgene plasmid # 170982 ; http://n2t.net/addgene:170982 ; RRID:Addgene_170982) -
For your References section:
CRISPR-Associated Transposase for Targeted Mutagenesis in Diverse Proteobacteria. Trujillo Rodriguez L, Ellington AJ, Reisch CR, Chevrette MG. ACS Synth Biol. 2023 Jun 27. doi: 10.1021/acssynbio.3c00065. 10.1021/acssynbio.3c00065 PubMed 37368499