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PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170978 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-2T
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Backbone manufacturerGE Healthcare Life Sciences
- Backbone size w/o insert (bp) 4304
- Total vector size (bp) 5850
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGlutathione-S-Transferase/LaG16/MNase
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SpeciesS. japonia/camelidae/Staphylococcus aureus
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Insert Size (bp)1546
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GenBank IDP00644
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pGEX 5`
- 3′ sequencing primer pGEX 3` (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRPN265NB-LaG16-MNase was a gift from Marc Timmers (Addgene plasmid # 170978 ; http://n2t.net/addgene:170978 ; RRID:Addgene_170978) -
For your References section:
greenCUT&RUN: Efficient Genomic Profiling of GFP-Tagged Transcription Factors and Chromatin Regulators. Koidl S, Timmers HTM. Curr Protoc. 2021 Oct;1(10):e266. doi: 10.1002/cpz1.266. 10.1002/cpz1.266 PubMed 34644460