pDONRp5-p2 SspB-mCherry-CShroom3
(Plasmid #170977)

• Purpose: C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
• Depositing Lab: Miki Ebisuya
• Publication: Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi: 10.1038/s41467-022-33115-0.

Backbone

• Vector backbone: pDONR221 p5-p2
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2597
• Total vector size (bp): 4988
• Vector type: Gateway cloning

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CShroom3
• Species: M. musculus (mouse)
• Insert Size (bp): 1254
• Mutation: Isoform X2, deleted aminoacids 1 - 1388
• GenBank ID: XP_006535031.1
• Entrez Gene: Shroom3 (a.k.a. AL022960, D5Ertd287, D5Ertd287e, Sh, Shr, Shrm, Shrm3)
• Tags / Fusion Proteins:
  • SspB (N terminal on backbone)
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 fwd: GTAAAACGACGGCCAGT
• 3′ sequencing primer: M13 rev: GTCATAGCTGTTTCCTG

Resource Information

• Supplemental Documents:
  • pDONRp5p2 SspB-mCherry-CShroom3.gbk
• A portion of this plasmid was derived from a plasmid made by: Original mShroom3 sequence was a gift from T. Nishimura, from M.Takeichi lab. SspB sequence was a gift from H. Janovjak lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2021.04.20.440475 for bioRxiv preprint.