EF1A_Hygro_P2A_RUNX1_N119I_Barcode
(Plasmid #170971)

• Purpose: Overexpression of RUNX1 N119I mutant protein.
• Depositing Lab: Prashant Mali
• Publication: Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi: 10.1016/j.celrep.2024.114436.

Backbone

• Vector backbone: EF1a_mCherry_P2A_Hygro_Barcode
• Backbone manufacturer: Prashant Mali (Addgene #120426)
• Backbone size w/o insert (bp): 9508
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RUNX1
• Alt name: AML1
• Species: H. sapiens (human)
• Insert Size (bp): 1359
• Mutation: N119I
• Entrez Gene: RUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
• Promoter: Ef1A

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTGTGTAGAAGTACTCGCCGATAGTG
• 3′ sequencing primer: TCTTGTCTTCGTTGGGAGTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.08.03.551876 for bioRxiv preprint.