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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17095 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+ (modified)
- Backbone size (bp) 4103
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Vector typexenopus/mammalian/avian/zebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pCS2+ has been modified by the deletion of the AC preceeding the T7 promoter addition of the sequence TTCTGCAGCCC immediately following the XbaI site. The PstI site added to CS2+ is encompassed in the sequence (CTGCAG).
Modifications were made by Robert Davis of Marc Kirshner's lab to improve the efficiency of the transcription from the T7 promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2P+ was a gift from Marc Kirschner (Addgene plasmid # 17095 ; http://n2t.net/addgene:17095 ; RRID:Addgene_17095)
Map uploaded by the depositor.
