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EF1A_Hygro_P2A_RUNX1_N69Y_Barcode
(Plasmid #170932)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170932 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EF1a_mCherry_P2A_Hygro_Barcode
  • Backbone manufacturer
    Prashant Mali (Addgene #120426)
  • Backbone size w/o insert (bp) 9508
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RUNX1
  • Alt name
    AML1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1359
  • Mutation
    N69Y
  • Entrez Gene
    RUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
  • Promoter Ef1A

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGTGTAGAAGTACTCGCCGATAGTG
  • 3′ sequencing primer TCTTGTCTTCGTTGGGAGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.08.03.551876 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF1A_Hygro_P2A_RUNX1_N69Y_Barcode was a gift from Prashant Mali (Addgene plasmid # 170932 ; http://n2t.net/addgene:170932 ; RRID:Addgene_170932)

  • For your References section:

    Interface-guided phenotyping of coding variants in the transcription factor RUNX1. Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, Hutter S, Haferlach T, Ideker T, Mali P, Carter H. Cell Rep. 2024 Jul 4;43(7):114436. doi: 10.1016/j.celrep.2024.114436. 10.1016/j.celrep.2024.114436 PubMed 38968069
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