pet21_TurboID_18A
(Plasmid
#170922)
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PurposeExpress TurboID_18A in Bl21
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170922 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePET21
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTurboID-18a
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer t7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pet21_TurboID_18A was a gift from Huan Bao (Addgene plasmid # 170922 ; http://n2t.net/addgene:170922 ; RRID:Addgene_170922) -
For your References section:
Developing Nanodisc-ID for label-free characterizations of membrane proteins. Bao H. Commun Biol. 2021 Apr 30;4(1):514. doi: 10.1038/s42003-021-02043-y. 10.1038/s42003-021-02043-y PubMed 33931748