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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepT7TS (based on pGEM4Z)
- Backbone size (bp) 3000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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GenBank IDAB255037
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' & 3' untranslated regions of Xenopus Beta-globin mRNA cloned into pGEM4Z. Insert size: 260 bp. Transcription: transcribe with T7 RNA polymerase. Cloning sites: Bgl II, EcoRV, SpeI
Xenbase has a nice description of this vector: http://www.xenbase.org/reagents/vectorAction.do?method=displayVectorSummary&vectorId=11536497&tabId=0
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7TS was a gift from Paul Krieg (Addgene plasmid # 17091 ; http://n2t.net/addgene:17091 ; RRID:Addgene_17091)
Map uploaded by the depositor.
