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ZE49 pSPORT1-Sp5-l
(Plasmid #17090)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17090 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSPORT1
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sp5l
  • Alt name
    Sp5-like
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2000
  • Entrez Gene
    sp5l (a.k.a. Spr2, YF-335, cb577, fc23b01, s, sb:cb57, sb:cb577, wu:fc23b01)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gilbert Weidinger, Ph.D

Zebrafish sp5-like (sp5l) cDNA (likely full-length) in pSPORT1.

Clone 27i2 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Expressed strongly in broad marginal region during gastrulation. Everywhere except head at bud, then rapidly declines to stay in tailbud and lateral mesoderm (or edge of neural plate).

For antisense: Asp718, SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE49 pSPORT1-Sp5-l was a gift from Randall Moon (Addgene plasmid # 17090 ; http://n2t.net/addgene:17090 ; RRID:Addgene_17090)
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