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Addgene

ZE48 Dvl3 pCS2P+
(Plasmid #17089)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17089 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CS2P+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dvl-3
  • Alt name
    Dishevelled 3
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    dvl3a (a.k.a. dvl3, wu:fb75f04, wu:fi22h02, wu:fp61b09)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dishevelled 3, cloned into Cla/EcoR1 sites.

Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Select clones with amp.

Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE48 Dvl3 pCS2P+ was a gift from Randall Moon (Addgene plasmid # 17089 ; http://n2t.net/addgene:17089 ; RRID:Addgene_17089)

  • For your References section:

    Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Waxman JS, Hocking AM, Stoick CL, Moon RT. Development. 2004 Dec. 131(23):5909-21. 10.1242/dev.01520 PubMed 15539487
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