ZE48 Dvl3 pCS2P+
(Plasmid #17089)

• Depositing Lab: Randall Moon
• Publication: Waxman et al Development. 2004 Dec. 131(23):5909-21.

Backbone

• Vector backbone: CS2P+
• Backbone size w/o insert (bp): 4100
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dvl-3
• Alt name: Dishevelled 3
• Species: D. rerio (zebrafish)
• Entrez Gene: dvl3a (a.k.a. dvl3, wu:fb75f04, wu:fi22h02, wu:fp61b09)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dishevelled 3, cloned into Cla/EcoR1 sites.

Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Select clones with amp.

Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.