pUPD+Red-F
(Plasmid #170874)

• Purpose: Phytobrick - Red-F Luciferase CDS
• Depositing Lab: Markita Landry
• Publication: Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.

Backbone

• Vector backbone: pUPD
• Backbone manufacturer: Diego Orzaez Lab
• Backbone size w/o insert (bp): 3057
• Vector type: Luciferase, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Red-F
• Alt name: Red mutant S286Y luciferase from Luciola curciata
• Species: Luciola curciata
• Insert Size (bp): 1648
• Mutation: p.S286Y Red mutant

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Esp3I (destroyed during cloning)
• 3′ cloning site: Esp3I (destroyed during cloning)
• 5′ sequencing primer: taatacgactcactatagg
• 3′ sequencing primer: gtcatagctgtttcctg

Resource Information

• Supplemental Documents:
  • 002 - pUPD+Red-F.gb
• A portion of this plasmid was derived from a plasmid made by: Original gene from https://doi.org/10.1016/j.cbpb.2006.06.001

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv.