ZE46 DeltaPGB Dvl2 pCS2P+
(Plasmid #17087)

• Depositing Lab: Randall Moon
• Publication: Waxman et al Development. 2004 Dec. 131(23):5909-21.

Backbone

• Vector backbone: CS2P+
• Backbone size w/o insert (bp): 4100
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dvl-2
• Alt name: Dishevelled 2
• Species: D. rerio (zebrafish)
• Mutation: DeltaPGB (deletes amino acids 231-252)
• Entrez Gene: dvl2 (a.k.a. sb:eu471, wu:fc05d12, wu:fo71e09, wu:fp54a02, zgc:55372)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dishevelled 2 deleted in amino acids 231-252, cloned into Cla/EcoR1 sites.

Notes for Use: For functional synthetic RNA cut ASP718, transcribe SP6.
Select clones with amp.

Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.