His-GFP-Granuphilin
(Plasmid
#170867)
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PurposeMammalian expression of His-GFP-Granuphilin.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170867 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClonetech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSynaptotagmin-like 4
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SpeciesH. sapiens (human)
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Entrez GeneSYTL4 (a.k.a. SLP4)
- Promoter CMV
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Tags
/ Fusion Proteins
- EGFP (N terminal on backbone)
- (His)6 (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-pArev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
His-GFP-Granuphilin was a gift from Justin Taraska (Addgene plasmid # 170867 ; http://n2t.net/addgene:170867 ; RRID:Addgene_170867) -
For your References section:
The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells. Prasai B, Haber GJ, Strub MP, Ahn R, Ciemniecki JA, Sochacki KA, Taraska JW. Nat Commun. 2021 Jun 25;12(1):3970. doi: 10.1038/s41467-021-24167-9. 10.1038/s41467-021-24167-9 PubMed 34172739
