His-Cavin1-GFP
(Plasmid
#170859)
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PurposeMammalian expression of His-Cavin1-GFP.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170859 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecaveolae associated 1 (Cavin1)
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SpeciesM. musculus (mouse)
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Entrez GeneCavin1 (a.k.a. 2310075E07Rik, Cav-p60, Cavin, Ptrf)
- Promoter CMV
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Tags
/ Fusion Proteins
- GFP (C terminal on backbone)
- (His)6 (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
His-Cavin1-GFP was a gift from Justin Taraska (Addgene plasmid # 170859 ; http://n2t.net/addgene:170859 ; RRID:Addgene_170859) -
For your References section:
The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells. Prasai B, Haber GJ, Strub MP, Ahn R, Ciemniecki JA, Sochacki KA, Taraska JW. Nat Commun. 2021 Jun 25;12(1):3970. doi: 10.1038/s41467-021-24167-9. 10.1038/s41467-021-24167-9 PubMed 34172739