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Addgene

His-Cavin1-GFP
(Plasmid #170859)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170859 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    caveolae associated 1 (Cavin1)
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cavin1 (a.k.a. 2310075E07Rik, Cav-p60, Cavin, Ptrf)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GFP (C terminal on backbone)
    • (His)6 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-Cavin1-GFP was a gift from Justin Taraska (Addgene plasmid # 170859 ; http://n2t.net/addgene:170859 ; RRID:Addgene_170859)
  • For your References section:

    The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells. Prasai B, Haber GJ, Strub MP, Ahn R, Ciemniecki JA, Sochacki KA, Taraska JW. Nat Commun. 2021 Jun 25;12(1):3970. doi: 10.1038/s41467-021-24167-9. 10.1038/s41467-021-24167-9 PubMed 34172739