pAAV-VTKD2-ChR2-mCherry
(Plasmid
#170845)
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PurposeAAV vector for Cre-dependent transgene expression of ChR2-mCherry in cortical interneurons under the control of the Dlx enhancer.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170845 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-VTKD2
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChR2-mCherry
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SpeciesSynthetic
- Promoter Dlx
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid also called pAAV-Dlx-Flex-ChR2-mCherry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-VTKD2-ChR2-mCherry was a gift from Jordane Dimidschstein (Addgene plasmid # 170845 ; http://n2t.net/addgene:170845 ; RRID:Addgene_170845) -
For your References section:
Hippocampal inputs engage CCK+ interneurons to mediate endocannabinoid-modulated feed-forward inhibition in the prefrontal cortex. Liu X, Dimidschstein J, Fishell G, Carter AG. Elife. 2020 Oct 9;9. pii: 55267. doi: 10.7554/eLife.55267. 10.7554/eLife.55267 PubMed 33034285