ZE42 myc-Dapper-1 pCS2P+
(Plasmid #17083)

• Depositing Lab: Randall Moon
• Publication: Waxman et al Development. 2004 Dec. 131(23):5909-21.

Backbone

• Vector backbone: CS2P+
• Backbone size w/o insert (bp): 4100
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dapper-1
• Alt name: Dpr1
• Species: D. rerio (zebrafish)
• Entrez Gene: dact1 (a.k.a. dpr1, frd1, frodo1, dapper1, MGC152821)
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dishevelled associated protein. tagged with c-myc epitope at amino terminus.

Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Select clones with amp.

Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.