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pCMV-GABRA1(A322D)-IRES2-EGFP
(Plasmid #170821)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170821 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-EGFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human GABAA receptor alpha1 subunit carrying A322D mutation
  • Alt name
    GABRA1 A322D
  • Species
    H. sapiens (human)
  • Mutation
    change Alanine 322 to Aspartate
  • GenBank ID
    NM_000806.5
  • Entrez Gene
    GABRA1 (a.k.a. DEE19, ECA4, EIEE19, EJM, EJM5)
  • Tag / Fusion Protein
    • IRES2-EGFP (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-GABRA1(A322D)-IRES2-EGFP was a gift from Tingwei Mu (Addgene plasmid # 170821 ; http://n2t.net/addgene:170821 ; RRID:Addgene_170821)

  • For your References section:

    Remodeling the endoplasmic reticulum proteostasis network restores proteostasis of pathogenic GABAA receptors. Fu YL, Han DY, Wang YJ, Di XJ, Yu HB, Mu TW. PLoS One. 2018 Nov 27;13(11):e0207948. doi: 10.1371/journal.pone.0207948. eCollection 2018. 10.1371/journal.pone.0207948 PubMed 30481215
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