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PET28a+_BrCas12b
(Plasmid #170819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170819 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28+
  • Backbone manufacturer
    Twist Bioscience
  • Backbone size w/o insert (bp) 5202
  • Total vector size (bp) 8601
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BrCas12b
  • Species
    Brevibacillus sp. SYP-B805
  • Insert Size (bp)
    3270
  • GenBank ID
    WP_165214399.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis, T7, Thrombin site (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PET28a+_BrCas12b was a gift from Piyush Jain (Addgene plasmid # 170819)

  • For your References section:

    A Thermostable Cas12b from Brevibacillus Leverages One-pot Detection of SARS-CoV-2 Variants of Concern. Nguyen LT, Macaluso NC, Pizzano BLM, Cash MN, Spacek J, Karasek J, Dinglasan RR, Salemi M, Jain PK. medRxiv 10.1101/2021.10.15.21265066
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