pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR
(Plasmid #170808)

• Purpose: PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance
• Depositing Lab: Kenneth Yamada
• Publication: Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30. doi: 10.1016/j.cell.2021.05.015. Epub 2021 Jun 15.

Backbone

• Vector backbone: pSLQ2842 (Addgene #84244)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GID1-VPR-IRES-HygR
• Species: A. thaliana (mustard weed), Synthetic; E. coli
• Insert Size (bp): 4281
• Mutation: Synonymous mutation in PYL1 to remove XhoI site (inherited from pSLQ2842; Addgene #84244)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 2

• Gene/Insert name: GAI-tagBFP-Sa_dCas9
• Species: A. thaliana (mustard weed), Synthetic; S. aureus
• Insert Size (bp): 4275
• Entrez Gene: GAI (a.k.a. AT1G14920, F10B6.34, F10B6_34, GAI PROTEIN, GIBBERELLIC ACID INSENSITIVE, RESTORATION ON GROWTH ON AMMONIA 2, RGA2)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint.