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pDY153: Flag(SUZ12) WT HDR plasmid
(Plasmid #170793)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170793 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    puc19
  • Total vector size (bp) 10600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUZ12
  • Species
    H. sapiens (human)
  • Entrez Gene
    SUZ12 (a.k.a. CHET9, IMMAS, JJAZ1)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was designed by Daniel Youmans but synthesized by GenScript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDY153: Flag(SUZ12) WT HDR plasmid was a gift from Thomas Cech (Addgene plasmid # 170793 ; http://n2t.net/addgene:170793 ; RRID:Addgene_170793)

  • For your References section:

    Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells. Youmans DT, Gooding AR, Dowell RD, Cech TR. Mol Cell. 2021 Feb 4;81(3):488-501.e9. doi: 10.1016/j.molcel.2020.11.044. Epub 2020 Dec 17. 10.1016/j.molcel.2020.11.044 PubMed 33338397
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