ZE38 pCS2P+ sp5l ORF
(Plasmid #17079)

• Depositing Lab: Randall Moon
• Publication: Thorpe et al Development. 2005 Apr. 132(8):1763-72.

Backbone

• Vector backbone: pCS2P+ (modified pCS2+)
• Backbone size w/o insert (bp): 4100
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sp5l ORF
• Alt name: sp5-like
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1100
• Entrez Gene: sp5l (a.k.a. Spr2, YF-335, cb577, fc23b01, s, sb:cb57, sb:cb577, wu:fc23b01)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Description: zebrafish sp5-like (sp5l) zinc-finger transcription factor of the SP5 family (cDNA clone 27i2 from the Wnt8 upregulated microarray screen) ORF only in pCS2P+. PCR amplified using primers 96 &97 from 27-i2 (pSPORT) clone and cloned EcoRI/XbaI into pCS2P+. Injection of high doses (>260 ng/�l corresponding to 200ng/�l #53.GFP) results in loss of eyes.

Species of Sequence Origin: zebrafish.

Notes for Use: For functional synthetic RNA, linearize with NotI, SP6 (1385bp RNA). Select clones with amp.
Reference: Development, Current Biology papers from the lab (2005).