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Addgene

pET-15b(+) Mortierella verticillate (delta prenylation motif)
(Plasmid #170788)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170788 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15B
  • Backbone manufacturer
    Novagen Inc., Madison, WI
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 6246
  • Modifications to backbone
    NA
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mortierella verticillate PRL
  • Species
    Mortierella verticillate
  • Insert Size (bp)
    555
  • Mutation
    Deleted nucleotides coding for 4 C-terminal amino acids
  • Promoter AmpR, lacI
  • Tag / Fusion Protein
    • N-terminal His-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pET-15b Fwd
  • 3′ sequencing primer pET-15b Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-15b(+) Mortierella verticillate (delta prenylation motif) was a gift from Kalle Gehring (Addgene plasmid # 170788 ; http://n2t.net/addgene:170788 ; RRID:Addgene_170788)
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