Rec-PhoA/CsgA
(Plasmid #170787)

• Purpose: Recombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinase
• Depositing Lab: Urartu Seker
• Publication: Ahan et al Chembiochem. 2019 Jul 15;20(14):1799-1809. doi: 10.1002/cbic.201800767. Epub 2019 May 15.

Backbone

• Vector backbone: pZA
• Backbone size w/o insert (bp): 1912
• Total vector size (bp): 4597
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: csgA
• Insert Size (bp): 453
• GenBank ID: NC_000913.3
• Entrez Gene: csgA (a.k.a. b1042, ECK1028)
• Promoter: Inverted proD with Bxb1recognition sites
• Tag / Fusion Protein:
  • HHHHHH

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CAAATCCGCCGCCCTAGA

Gene/Insert 2

• Gene/Insert name: phoA
• Insert Size (bp): 1416
• GenBank ID: NC_000913.3
• Entrez Gene: phoA (a.k.a. b0383, ECK0378, psiA)
• Promoter: Inverted proD with TP901 recognition site

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gaaaagtgccacctgACGTC

Resource Information

• Supplemental Documents:
  • rec-gate-alp-and-csgahis.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry