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Addgene

pGEX-6P1 Danio rerio CBS-pair domain of CNNM4
(Plasmid #170785)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170785 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    Amersham-Pharmacia
  • Backbone size w/o insert (bp) 4984
  • Total vector size (bp) 5428
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBS from CNNM4
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    474
  • Mutation
    NA
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX Fwd
  • 3′ sequencing primer pGEX Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-6P1 Danio rerio CBS-pair domain of CNNM4 was a gift from Kalle Gehring (Addgene plasmid # 170785 ; http://n2t.net/addgene:170785 ; RRID:Addgene_170785)
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