pcDNA3-ZnRed
(Plasmid #170782)

• Purpose: cytosolic ZnRed zinc sensor with 166 nM and 20 uM Kd
• Depositing Lab: Huiwang Ai
• Publication: Chen et al Anal Chem. 2016 Sep 20;88(18):9029-36. doi: 10.1021/acs.analchem.6b01653. Epub 2016 Aug 29.

Backbone

• Vector backbone: pcDNA3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ZnRed
• Species: Synthetic
• Insert Size (bp): 909
• Mutation: K255N
• GenBank ID: KU867815
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

When selecting zinc biosensors for your particular application, please pay attention to the Kd. The Kd of the sensor has to match the concentration range of your particular experiments. Please note that the K255N mutation in ZnRed does not alter plasmid function.