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Addgene

pcDNA3-ZnRed
(Plasmid #170782)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170782 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZnRed
  • Species
    Synthetic
  • Insert Size (bp)
    909
  • Mutation
    K255N
  • GenBank ID
    KU867815
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

When selecting zinc biosensors for your particular application, please pay attention to the Kd. The Kd of the sensor has to match the concentration range of your particular experiments. Please note that the K255N mutation in ZnRed does not alter plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-ZnRed was a gift from Huiwang Ai (Addgene plasmid # 170782 ; http://n2t.net/addgene:170782 ; RRID:Addgene_170782)
  • For your References section:

    Single Fluorescent Protein-Based Indicators for Zinc Ion (Zn2+). Chen Z, Ai HW. Anal Chem. 2016 Sep 20;88(18):9029-36. doi: 10.1021/acs.analchem.6b01653. Epub 2016 Aug 29. 10.1021/acs.analchem.6b01653 PubMed 27539450