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Addgene

lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC
(Plasmid #170772)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170772 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Backbone manufacturer
    pTwist
  • Backbone size w/o insert (bp) 3
  • Total vector size (bp) 3760
  • Modifications to backbone
    encodes promoter, ribosome binding site, lacI structural gene, tac promoter, lac operon, BsaI-EcoRV-BsaI multiple cloning site

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lacI
  • Species
    Synthetic
  • Insert Size (bp)
    1082
  • Promoter lacI promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MfeI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer part sequenced in different plasmid prior to subcloning
  • 3′ sequencing primer part sequenced in different plasmid prior to subcloning
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was designed to test Golden Gate assembly protocols

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC was a gift from Ichiro Matsumura (Addgene plasmid # 170772 ; http://n2t.net/addgene:170772 ; RRID:Addgene_170772)

  • For your References section:

    Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases. Matsumura I. Biotechniques. 2022 May;72(5):185-193. doi: 10.2144/btn-2021-0083. Epub 2022 Mar 8. 10.2144/btn-2021-0083 PubMed 35255734
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