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Addgene

BsaI-tagRFP-BsaI-pUC-kan
(Plasmid #170771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Backbone manufacturer
    GenScript
  • Backbone size w/o insert (bp) 2579
  • Total vector size (bp) 2730

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tagRFP
  • Species
    Synthetic
  • Insert Size (bp)
    695
  • Promoter none

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer part sequenced by manufacturer (GenScript)
  • 3′ sequencing primer part sequenced by manufacturer (GenScript)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The tagRFP gene was synthesized, cloned and sequenced by GenScript.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was designed to test Golden Gate assembly protocols

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BsaI-tagRFP-BsaI-pUC-kan was a gift from Ichiro Matsumura (Addgene plasmid # 170771 ; http://n2t.net/addgene:170771 ; RRID:Addgene_170771)

  • For your References section:

    Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases. Matsumura I. Biotechniques. 2022 May;72(5):185-193. doi: 10.2144/btn-2021-0083. Epub 2022 Mar 8. 10.2144/btn-2021-0083 PubMed 35255734
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