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Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA
(Plasmid #170767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC
  • Backbone manufacturer
    EMD Millipore/Novagen
  • Backbone size w/o insert (bp) 1724
  • Total vector size (bp) 6436
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    OmniMax 2
  • Growth instructions
    Please use OmniMax 2 bacterial strain for expression.
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    M.XbaI
  • Species
    Synthetic
  • Insert Size (bp)
    1271
  • GenBank ID
  • Promoter rhamnose

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer part sequenced in different plasmid prior to subcloning
  • 3′ sequencing primer part sequenced in different plasmid prior to subcloning
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    M.EcoRI
  • Species
    Synthetic
  • Insert Size (bp)
    980
  • Promoter rhamnose

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer part sequenced in different plasmid prior to subcloning
  • 3′ sequencing primer part sequenced in different plasmid prior to subcloning
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    M.SalI
  • Species
    Synthetic
  • Insert Size (bp)
    1763
  • Promoter rhamnose

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer part sequenced in different plasmid prior to subcloning
  • 3′ sequencing primer part sequenced in different plasmid prior to subcloning
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA was a gift from Ichiro Matsumura (Addgene plasmid # 170767 ; http://n2t.net/addgene:170767 ; RRID:Addgene_170767)

  • For your References section:

    Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases. Matsumura I. Biotechniques. 2022 May;72(5):185-193. doi: 10.2144/btn-2021-0083. Epub 2022 Mar 8. 10.2144/btn-2021-0083 PubMed 35255734
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