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Addgene

A99-ELP
(Plasmid #170760)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170760 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET25b+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5547
  • Total vector size (bp) 5977
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    A99-ELP
  • Species
    Synthetic
  • Insert Size (bp)
    518
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    A99-ELP was a gift from Andrew MacKay (Addgene plasmid # 170760 ; http://n2t.net/addgene:170760 ; RRID:Addgene_170760)

  • For your References section:

    Synergy between Laminin-Derived Elastin-like Polypeptides (LELPs) Optimizes Cell Spreading. Truong AT, Lee SJ, Hamada K, Kiyomi A, Guo H, Yamada Y, Kikkawa Y, Okamoto CT, Nomizu M, MacKay JA. Biomacromolecules. 2024 Jul 8;25(7):4001-4013. doi: 10.1021/acs.biomac.4c00144. Epub 2024 May 30. 10.1021/acs.biomac.4c00144 PubMed 38814168
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