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pUC19_dTAG-KANSL3-targeting
(Plasmid #170755)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170755 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 4584
  • Vector type
    Mouse Targeting
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BSD-P2A-2xHA-dTAG
  • Alt name
    BSD-P2A-2xHA-FKBP12(F36V)
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    1896
  • Mutation
    Phenylalanine 36 to valine in FKBP12
  • Tag / Fusion Protein
    • BSD-P2A-2xHA-dTAG

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer M13/pUC19 reverse
  • 3′ sequencing primer M13/pUC19 forward
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    the BSD-P2A-2xHA-dTAG cassette was amplified by PCR from pCRIS-PITChv2-BSD-dTAG BRD4 vector (addgene #91792)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_dTAG-KANSL3-targeting was a gift from Kristian Helin (Addgene plasmid # 170755 ; http://n2t.net/addgene:170755 ; RRID:Addgene_170755)

  • For your References section:

    Generation of locus-specific degradable tag knock-ins in mouse and human cell lines. Damhofer H, Radzisheuskaya A, Helin K. STAR Protoc. 2021 Jun 2;2(2):100575. doi: 10.1016/j.xpro.2021.100575. eCollection 2021 Jun 18. 10.1016/j.xpro.2021.100575 PubMed 34151298
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