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pSUMO human-cPLA2-C2-domain
(Plasmid #170752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUMO
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLA2G4A-C2 domain
  • Alt name
    cPLA2_-C2 domain
  • Species
    H. sapiens (human)
  • Entrez Gene
    PLA2G4A (a.k.a. GURDP, PLA2G4, cPLA2, cPLA2-alpha)
  • Tag / Fusion Protein
    • SUMO (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUMO human-cPLA2-C2-domain was a gift from Rhoderick Brown (Addgene plasmid # 170752 ; http://n2t.net/addgene:170752 ; RRID:Addgene_170752)

  • For your References section:

    Structural basis of phosphatidylcholine recognition by the C2-domain of cytosolic phospholipase A2alpha. Hirano Y, Gao YG, Stephenson DJ, Vu NT, Malinina L, Simanshu DK, Chalfant CE, Patel DJ, Brown RE. Elife. 2019 May 3;8. pii: 44760. doi: 10.7554/eLife.44760. 10.7554/eLife.44760 PubMed 31050338
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