pFLAG GLTPD2
(Plasmid
#170745)
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PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170745 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFLAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGLTPD2
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SpeciesH. sapiens (human)
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MutationV49F, V152A and D209E- Please see depositor comments
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Entrez GeneGLTPD2
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note GLTPD2 contains 3 mutations V49F, V152A and D209E compared to NCBI reference NP_001014985.3. However Uniprot website mentions these three 'mutations' as sequence conflicts. Depositor confirms plasmid is functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLAG GLTPD2 was a gift from Rhoderick Brown (Addgene plasmid # 170745 ; http://n2t.net/addgene:170745 ; RRID:Addgene_170745)