pSUMO FAPP2-GLTPH
(Plasmid
#170739)
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PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170739 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSUMO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePLEKHA8-GLTPH
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Alt nameFAPP2-GLTPH
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SpeciesH. sapiens (human)
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Entrez GenePLEKHA8 (a.k.a. FAPP2)
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Tag
/ Fusion Protein
- SUMO (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSUMO FAPP2-GLTPH was a gift from Rhoderick Brown (Addgene plasmid # 170739 ; http://n2t.net/addgene:170739 ; RRID:Addgene_170739) -
For your References section:
Structural analyses of 4-phosphate adaptor protein 2 yield mechanistic insights into sphingolipid recognition by the glycolipid transfer protein family. Ochoa-Lizarralde B, Gao YG, Popov AN, Samygina VR, Zhai X, Mishra SK, Boldyrev IA, Molotkovsky JG, Simanshu DK, Patel DJ, Brown RE, Malinina L. J Biol Chem. 2018 Oct 26;293(43):16709-16723. doi: 10.1074/jbc.RA117.000733. Epub 2018 Sep 11. 10.1074/jbc.RA117.000733 PubMed 30206120