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Addgene

pET30 W96A-GLTP
(Plasmid #170734)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170734 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET30 Xa/LIC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    W96A-GLTP
  • Species
    H. sapiens (human)
  • Mutation
    W96A
  • Entrez Gene
    GLTP
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30 W96A-GLTP was a gift from Rhoderick Brown (Addgene plasmid # 170734 ; http://n2t.net/addgene:170734 ; RRID:Addgene_170734)
  • For your References section:

    Structural basis for glycosphingolipid transfer specificity. Malinina L, Malakhova ML, Teplov A, Brown RE, Patel DJ. Nature. 2004 Aug 26;430(7003):1048-53. doi: 10.1038/nature02856. 10.1038/nature02856 PubMed 15329726