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pBS-ACTB-C200-GFPfr2
(Plasmid #170726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170726 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II SK (+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2898
  • Total vector size (bp) 3295
  • Vector type
    HR donor vector for human ACTB gene.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Donor sequence to ACTB gene for HR with the revised marker sequence
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer aattaaccctcactaaagg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Using this plasmid as a substitute for pBS-ACTB-C200-GFPfr1, less non-specific amplification is observed in the downstream qPCR.
The following primer set can be used to detect the HR product.
GFPrev2-F: 5'-CTTCAAGATCCGCCACAACGT-3'
ACTB-common-R1: 5'-GTGCAATCAAAGTCCTCGGC-3'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-ACTB-C200-GFPfr2 was a gift from Natsuko Chiba (Addgene plasmid # 170726 ; http://n2t.net/addgene:170726 ; RRID:Addgene_170726)
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