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pDONR207-MUM2sp-Citrine-ADPG2
(Plasmid #170725)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170725 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR207
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5585
  • Total vector size (bp) 5474
  • Vector type
    Gateway Donor vector / entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For selection use 7 ug/ml Gentamicin
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2
  • Alt name
    ADPG2
  • Alt name
    At2g41850
  • Alt name
    Q8RY29 (Uniprot)
  • Species
    A. thaliana (mustard weed)
  • GenBank ID
    AC002339
  • Entrez Gene
    PGAZAT (a.k.a. AT2G41850, ADPG2, ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2, T11A7.5, T11A7_5, polygalacturonase abscission zone A. thaliana)
  • Tag / Fusion Protein
    • Citrine tag (714bp) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site AatII (not destroyed)
  • 5′ sequencing primer TAACGCTAGCATGGATCTC
  • 3′ sequencing primer GTAACATCAGAGATTTTGAGACA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use with Three-way Multi-site Gateway Cloning (Invitrogen).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR207-MUM2sp-Citrine-ADPG2 was a gift from George Haughn (Addgene plasmid # 170725 ; http://n2t.net/addgene:170725 ; RRID:Addgene_170725)

  • For your References section:

    Pectin Modification in Seed Coat Mucilage by In Vivo Expression of Rhamnogalacturonan-I- and Homogalacturonan-Degrading Enzymes. McGee R, Dean GH, Wu D, Zhang Y, Mansfield SD, Haughn GW. Plant Cell Physiol. 2021 Jun 1. pii: 6290394. doi: 10.1093/pcp/pcab077. 10.1093/pcp/pcab077 PubMed 34059917
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