Skip to main content
Addgene

pDONR207-MUM2sp-RglB
(Plasmid #170721)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 170721 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pDONR207
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5585
  • Total vector size (bp) 5400
  • Vector type
    Gateway Donor vector / entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For selection use 7 ug/ml Gentamicin
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rhamnogalacturonan lyase B
  • Alt name
    RglB
  • Alt name
    AN6395.2
  • Alt name
    Q5AZ85 (Uniprot)
  • Species
    Aspergillus nidulans
  • Insert Size (bp)
    1925
  • GenBank ID
    DQ490501

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site AatII (not destroyed)
  • 5′ sequencing primer TAACGCTAGCATGGATCTC
  • 3′ sequencing primer GTAACATCAGAGATTTTGAGACA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use with Three-way Multi-site Gateway Cloning (Invitrogen).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR207-MUM2sp-RglB was a gift from George Haughn (Addgene plasmid # 170721 ; http://n2t.net/addgene:170721 ; RRID:Addgene_170721)

  • For your References section:

    Pectin Modification in Seed Coat Mucilage by In Vivo Expression of Rhamnogalacturonan-I- and Homogalacturonan-Degrading Enzymes. McGee R, Dean GH, Wu D, Zhang Y, Mansfield SD, Haughn GW. Plant Cell Physiol. 2021 Jun 1. pii: 6290394. doi: 10.1093/pcp/pcab077. 10.1093/pcp/pcab077 PubMed 34059917
Related items: