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ZE31 Prickle-1-pCS2P+
(Plasmid #17072)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CS2P+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Prickle-1
  • Alt name
    pk1
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    prickle1a (a.k.a. fk71b09, pk1, prickle1, wu:fk71b09)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Zebrafish prickle-1 (full-length ORF), cloned into the BamH1 and EcoR1 sites of CS2P+.

Species of Sequence Origin: zebrafish.

Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp.

Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE31 Prickle-1-pCS2P+ was a gift from Randall Moon (Addgene plasmid # 17072 ; http://n2t.net/addgene:17072 ; RRID:Addgene_17072)

  • For your References section:

    Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT. Curr Biol. 2003 Apr 15. 13(8):680-5. 10.1016/S0960-9822(03)00240-9 PubMed 12699626
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