pFU-GW-sMYH6-mNeon-TNNT2-mScarlet
(Plasmid
#170712)
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Purposedual fluorescent reporter for MYH6 (short) and TNNT2 promoters
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFUGW
- Backbone size w/o insert (bp) 9941
- Total vector size (bp) 12247
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namemNeonGreen
- Promoter aMHC
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer aMHC_Fwd
- 3′ sequencing primer mNeon_Rev (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namemScarlet
- Promoter TNNT2
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer TNNT2_Fwd
- 3′ sequencing primer mNeon_Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFU-GW-sMYH6-mNeon-TNNT2-mScarlet was a gift from Paul Burridge (Addgene plasmid # 170712 ; http://n2t.net/addgene:170712 ; RRID:Addgene_170712) -
For your References section:
A novel transcription factor combination for direct reprogramming to a spontaneously contracting human cardiomyocyte-like state. Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW. J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 10.1016/j.yjmcc.2023.06.005. 10.1016/j.yjmcc.2023.06.005 PubMed 37421991
Map uploaded by the depositor.
