ZE29 Stbm-deltaN-6myc-pCS2+
(Plasmid #17071)

• Depositing Lab: Randall Moon
• Publication: Park et al Nat Cell Biol. 2002 Jan;4(1):20-5.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4100
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Stbm deltaN
• Alt name: strabismus
• Species: D. rerio (zebrafish)
• Insert Size (bp): 850
• Mutation: amino acids 240-526 only
• Entrez Gene: vangl2 (a.k.a. stbm, tri, vang)
• Tag / Fusion Protein:
  • Myc6 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Zebrafish strabismus amino acids 240-526 with 6 c-myc epitopes at N-terminus; EcoRI-Xba1 insert.

For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase.

Plasmid History:
Species of Sequence Origin: Zebrafish.
Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002.