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ZE27 Stbm-deltaPBM-6myc-pCS2+
(Plasmid #17069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17069 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Stbm deltaPBM
  • Alt name
    strabismus
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1500
  • Mutation
    amino acids 1-516 only
  • Entrez Gene
    vangl2 (a.k.a. stbm, tri, vang)
  • Tag / Fusion Protein
    • Myc6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Zebrafish strabismus amino acids 1-516 with 6 c-myc epitopes at C-terminus. BamHI-Cla1 insert.

For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase.

Plasmid History:
Species of Sequence Origin: Zebrafish.
Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE27 Stbm-deltaPBM-6myc-pCS2+ was a gift from Randall Moon (Addgene plasmid # 17069)

  • For your References section:

    The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos. Park M, Moon RT. Nat Cell Biol. 2002 Jan;4(1):20-5. 10.1038/ncb716 PubMed 11780127
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