tetO-ETS2
(Plasmid #170685)

• Purpose: doxycycline-inducible overexpression of ETS2
• Depositing Lab: Paul Burridge
• Publication: Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 10.1016/j.yjmcc.2023.06.005.

Backbone

• Vector backbone: FU-tetO-Gateway
• Backbone manufacturer: addgene plasmid #43914
• Backbone size w/o insert (bp): 8600
• Total vector size (bp): 10017
• Modifications to backbone: ETS2 ORF added to plasmid using Gateway cloning
• Vector type: Mammalian Expression, Lentiviral ; Doxycycline Inducible

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ETS2
• Species: H. sapiens (human)
• Insert Size (bp): 1410
• Entrez Gene: ETS2 (a.k.a. ETS2IT1)
• Promoter: TRE promoter, Tet-ON

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: LNCX; T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ETS2 cDNA was obtained from Pumin Zhang (Addgene plasmid # 28128 ; http://n2t.net/addgene:28128 ; RRID:Addgene_28128)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated using Gateway cloning. attB sites were added to ETS2 via PCR reaction. Resulting fragements were recombined with pDONR221 to generate an entry clone. LR reaction was performed using the destination vector FU-tetO-Gateway (Addgene Plasmid #19778).