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Addgene

tetO-AKT1
(Plasmid #170682)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170682 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FU-tetO-Gateway
  • Backbone manufacturer
    addgene plasmid #43914
  • Backbone size w/o insert (bp) 8600
  • Total vector size (bp) 10050
  • Modifications to backbone
    AKT1 ORF added to plasmid using Gateway cloning
  • Vector type
    Mammalian Expression, Lentiviral ; Doxycycline Inducible

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AKT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1443
  • GenBank ID
    NM_001014431
  • Entrez Gene
    AKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  • Promoter TRE promoter, Tet-ON

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pWZL Neo Myr Flag AKT1 (Addgene Plasmid #20422)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated using Gateway cloning. attB sites were added to AKT1 via PCR reaction using pWZL-Neo-Myr-Flag-AKT1 (Addgene Plasmid #20422) as a template. Resulting fragements were recombined with pDONR221 to generate an entry clone. LR reaction was performed using the destination vector FU-tetO-Gateway (Addgene Plasmid #19778).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tetO-AKT1 was a gift from Paul Burridge (Addgene plasmid # 170682 ; http://n2t.net/addgene:170682 ; RRID:Addgene_170682)

  • For your References section:

    A novel transcription factor combination for direct reprogramming to a spontaneously contracting human cardiomyocyte-like state. Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW. J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 10.1016/j.yjmcc.2023.06.005. 10.1016/j.yjmcc.2023.06.005 PubMed 37421991
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