pAPV-mEmerald-DsRed2-ER-5
(Plasmid
#170681)
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PurposeExpresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDendra2-C
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4026
- Total vector size (bp) 5469
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAPV-mEmerald-DsRed2-ER-5
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SpeciesSynthetic
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Insert Size (bp)1494
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAPV-mEmerald-DsRed2-ER-5 was a gift from Ke Xu (Addgene plasmid # 170681 ; http://n2t.net/addgene:170681 ; RRID:Addgene_170681) -
For your References section:
SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport. Yan R, Chen K, Wang B, Xu K. Dev Cell. 2022 Feb 28;57(4):512-525.e8. doi: 10.1016/j.devcel.2021.12.018. Epub 2022 Jan 19. 10.1016/j.devcel.2021.12.018 PubMed 35051356